Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA

Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA

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The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of: History and background Principles and theory Equipment and reagents Protocols Troubleshooting Applications Improvements Results Comparisons with other methods Future prospects and developments This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.Key. Reagents. and. Consumables. for. Minisatellite. Analysis. Note: ... 3.4.1 Preparation of DNA Fingerprints and DNA Profiles from Blood (For detailed protocols on other types of samples see ... Incubate for 1 h at 56AdC. Extract with 120 ul of phenol/chloroform/isocmyl/alcohol solution and remove 300 to 400 pul of theanbsp;...


Title:Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA
Author: Graham R. Taylor
Publisher:CRC Press - 1997-01-24
ISBN-13:

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